Hearts of Oak have been paired with Liberty Professionals while Asante Kotoko will face Berkwai Youth FC in the MTN FA Cup round 64 after the draw was held on Thursday.
Hearts of Oak who have won the competition a record 10 times must be at their best to overcome their regional rivals and reach round of 32.
Kotoko, on the other hand, are clear favourites against the second-tier side. Kotoko and Berkwai met at the same stage in 2017 with the Porcupine Warriors winning on penalties after a 1-1 stalemate in regulation time. Kotoko went on to win the tournament, beating Hearts of Oak 2-1 in the final in Tamale.
Other exciting fixtures include Aboi United FC versus Samartex 1996.
Two time winners Medeama SC will face Sekondi Eleven Wise – with 1985 champions Okwaku United set to face Division Two side Kade United FC.
Matches in the Round of 64 are scheduled for Friday, May 28 to Monday, May 31, 2021.
See full draw below
Great Olympics vs Charity Stars FC
Tema Youth FC vs Inter Allies FC
Accra City Stars FC vs Third World FC
Hearts of Oak vs Liberty Professionals
Legon Cities vs Uncle T FC
Attram De Visser vs Mighty Jets FC
Okyeman Planners FC vs Young Wise FC
Kade United FC vs Okwawu United
Phar Rangers vs Dreams FC
Kintampo Top Talents FC vs Mighty Royals FC
Aduana Stars vs Eleven Wonders
Kato Freedom Fighters vs Bechem United
Kenyasi New Dreams FC vs Sunyani Reformers
Young Apostles FC vs TECHIMAN City/Unity FC
Berekum Chelsea vs Bofoakwa Tano FC
WAFA FC vs Akatsi Allstars FC
Hearts of Lions FC vs Bebeto FC
Wassaman FC vs Asokwa Deportivo
River Plate Athletic Club vs Thunderbolt FC
BYFA vs Asante Kotoko
King Faisal vs Ashantigold
Kintampo FC vs Bolga Soccer Masters
Real 24 hours FC vs Steadfast FC
Paga Crocodiles vs Sombo Freedom Stars
RTU vs Tamale City
Aboi Young Stars vs Samartex
Sekondi Eleven Wise vs Medeama SC
Sekondi Hasaacas vs Karela United FC