The government in Havana says those to be freed include inmates due for conditional release next year, as well as a number of foreign prisoners.
Those found guilty of crimes against state security will not be eligible, seemingly ruling out several high-profile political prisoners.
The Pope will make a three-day visit to the island next week.
According to Cuba’s state-run newspaper Granma, prisoners were chosen for early release depending on the severity of their crimes, amount of time served, and good behaviour.
Among those to be freed are a number of inmates over 60, prisoners under 20 years old with no prior criminal records and inmates with chronic health conditions.
No prisoners with sentences for murder, other violent crimes or drug trafficking will be released, the paper said.
Pope Francis is expected to meet with Fidel Castro if the former Cuban leader’s health allows.
The 78-year-old Argentine pontiff is credited with helping bring about last December’s diplomatic thaw after the decades-old enmity between communist Cuba and the US.
He was directly involved in organising a series of secret diplomatic meetings between the two sides.
The Roman Catholic Church has maintained relations with Havana since the revolution and Pope Francis himself is no stranger to Cuba. He was there during Pope Benedict XVI’s visit in 2012 and is the author of a book on Cuba.